![]() ![]() For a combination of porin B, autotransporter E, and hypothetical protein B, with an OD 450 cutoff of 0♳4, sensitivity was 76♹% (56♴–91♰) and specificity was 93♸% (86♹–97♷) for all Peruvian patient samples. ![]() For the combination of porin B and autotransporter E, with optical density measured at 450 nm (OD 450) cutoff value of 0♲9, sensitivity was 80♸% (95% CI 60♷–93♵) and specificity was 94♸% (88♳–98♳) for all Peruvian patient samples. By using serum samples of Peruvian patients with Carrión's disease and of healthy German blood donors, we identified three antigens (porin B, autotransporter E, and hypothetical protein B) as suitable immunodominant antigens, and we applied them in a diagnostic ELISA using two different antigen combinations (porin B plus autotransporter E and porin B plus autotransporter E plus hypothetical protein B). Of these 21 antigens, 14 were found to be immunoreactive. 21 potentially immunodominant proteins were identified and recombinantly expressed, and their reactivity was assessed with immunoblotting and line blots. It can be used to analyze, graph, and present scientific data. We used serum samples obtained between Dec 23, 1990, and May 5, 2018, from 26 Peruvian patients with B bacilliformis infections and serum samples taken between Aug 28 and Aug 31, 2020, from 96 healthy German blood donors. GraphPad Prism combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. ![]()
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